工业微生物课题组

(Microbial Research Group)



成员(Members):

学术带头人:肖梓军教授/博导
Leader: Prof. Zijun Xiao

Email: zjxiao@upc.edu.cn
Phone: 0086-15806525897
2007
7月–至今,中国石油大学(华东)化学工程学院生物工程与技术中心教师
2012
6月–20135月,英国曼彻斯特大学访问学者
2002
9月–20076月,山东大学微生物学专业博士
2000
7月–20028月,济南啤酒集团总公司啤酒发酵车间工人
1996
9月–20006月,山东轻工业学院发酵工程专业本科

成员:赵静宜讲师
Staff: Dr. Jing-yi Zhao

Email: jyzhao@upc.edu.cn
2008
7月–至今,中国石油大学(华东)化学工程学院生物工程与技术中心教师
2003
9月–20086月,山东大学微生物学专业博士
1999
9月–20036月,山东大学制药工程专业本科

在读研究生:
MSc students:

钟昊璇    王卓   马小龙    郑景泽    孟哲     申杰

Haoxuan Zhong   Zhuo Wang   Xiaolong Ma   Jingze Zheng  Zhe Meng Jie Shen

      

        

已毕业研究生:田莉、马玲燕、董方卉、梁文龙、顾如林、赵玲、王霖慧、赵书梅、侯孝元、牟晓东、张瑜、朱贤琨、王相明、黄运玲、霍方方、亓瑞倩、乔树苓
Graduated students: Li Tian, Lingyan Ma, Fanghui Dong, Wenlong Liang, Rulin Gu, Ling Zhao, Linhui Wang, Shu-mei Zhao, Xiaoyuan Hou, Xiao-dong Mu, Yu Zhang, Xiankun Zhu, Xiangming Wang, Yunling Huang, Fangfang Huo, Ruiqian Qi, Shuling Qiao

研究方向(Researches):

本课题组目前主要研究工业微生物和药食同源功效物质,围绕新菌种、新基因、功能酶及新型活性产物,借助现代分子生物学与微生物组学等手段,开发代谢控制发酵应用技术

Fig. 1The green process of tetramethylpyrazine (TMP) production from glucose by microbiotechnology.



Fig. 2 The dependence of tetramethylpyrazine (Z) on acetoin (X) and ammonium (Y) in Chinese vinegars.

Fig. 3 Total ion chromatogram overlaid with the mass spectra of peak 12.32 (A) and 5-Hydroxy-γ-decalactone from the NIST library (B).

Fig. 4 Myroides sp. ZB35 colonies (30°C, 12 h) on LB plates and its scanning electron image.

Fig. 5 Solid-state fermentative production of aroma esters by Myroides sp. ZB35.


Fig. 6 Acetoin biosynthesis pathway and gene operons of typical acetoin producing species.

Fig. 7 Simplified phylogenetic tree based on typical amino acid sequences of putative acetoin and/or 2-acetolactate biosynthesis enzymes.


Fig. 8 Typical kinetic process of acetoin biosynthesis from glucose by Bacillus subtilis CICC 10211.

Fig. 9 Generation of acetoin and its derivatives in foods.



Fig. 10 Generation pathways of acetoin and its derivatives.

主要承担项目(Grants):
1.
国家自然科学基金面上项目:巴氏醋杆菌乙偶姻代谢机理研究

2.国家自然科学基金面上项目:基于基因组学的新型地芽孢杆菌合成乙偶姻及其衍生物研究

3.国家自然科学基金青年项目:细菌中2,3-丁二醇/乙偶姻/双乙酰分解代谢机理及未知蛋白AcoX功能研究

4.国家自然科学基金青年项目:水环境细菌中喹诺酮耐药基因qnr的传播机制研究

5.山东省中青年科学家科研奖励基金项目:巴氏醋杆菌乙酰乳酸合成酶基因alsS的功能和调控机制研究

6.山东省自然科学基金青年项目:一株罕见的拥有乙偶姻代谢途径的嗜热菌

代表性论文(Publications):

  1. Zijun Xiao*, Lingyan Ma, Jing-yi Zhao, Haoxuan Zhong, Yawei Sun (2018) 5-Hydroxy-γ-decalactone production by Bacillus sp. 1s-1 and its complete genome sequence. Journal of Biotechnology, 286:68-70

  2. Zijun Xiao*, Ling Zhao, Li Tian, Linhui Wang, Jing-yi Zhao (2018) GC-FID determination of tetramethylpyrazine and acetoin in vinegars and quantifying the dependence of tetramethylpyrazine on acetoin and ammonium. Food Chemistry, 239:726-732

  3. Zijun Xiao, Rulin Gu, Jing-yi Zhao, Linhui Wang, Xiaoyuan Hou, Hu Zhu* (2017) Hydroxy-pentanones production by Bacillus sp. H15-1 and its complete genome sequence. Journal of Biotechnology, 259:26-29

  4. Zijun Xiao*, Rulin Gu, Xiaoyuan Hou, Jing-yi Zhao, Hu Zhu and Jian R. Lu* (2017) Non-sterilized fermentative production of acetoin with 2,3-butanediol as a main byproduct from maize hydrolysate by a newly isolated thermophilic Bacillus strain. Journal of Chemical Technology and Biotechnology, 92: 2845–2852

  5. Zijun Xiao*, Wenlong Liang, Xiankun Zhu, Jing-yi Zhao (2017) Solid-state fermentative production of aroma esters by Myroides sp. ZB35 and its complete genome sequence. Journal of Biotechnology, 245:28-33

  6. Jing-yi Zhao, Shu-mei Zhao, Xiao-dong Mu, Zijun Xiao* (2017) Genetic characterization of plasmid-mediated quinolone resistance gene qnrS2 in Pseudoalteromonas and Shewanella isolates from seawater. FEMS Microbiology Letters, 364:fnw295

  7. Jing-yi Zhao, Xiao-dong Mu, Yuan-qi Zhu, Lijun Xi, Zijun Xiao* (2015) Identification of an integron containing the quinolone resistance gene qnrA1 in Shewanella xiamenensis. FEMS Microbiology Letters, 362:fnv146

  8. Zijun Xiao *, Jian R. Lu* (2014) Strategies for enhancing fermentative production of acetoin: a review. Biotechnology Advances, 32(2):492-503

  9. Zijun Xiao*, Xiaoyuan Hou, Lijun Xi, Jing-yi Zhao (2014) Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate. Biotechnology Biofuels, 7:106

  10. Zijun Xiao*, Jian R. Lu* (2014) Generation of acetoin and its derivatives in foods. Journal of Agricultural and Food Chemistry, 62(28):6487-6497

  11. Zijun Xiao*, Xiangming Wang, Yunling Huang, Fangfang Huo, Xiankun Zhu, Lijun Xi, Jian R. Lu* (2012) Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain. Biotechnol Biofuels, 5:88

  12. Zijun Xiao, Fangfang Huo, Yunling Huang, Xiankun Zhu, Jian R. Lu * (2012) A novel 2,3-xylenol-utilizing Pseudomonas isolate capable of degrading multiple phenolic compounds. Bioresource Technology, 104:59-64

  13. Zijun Xiao, Chuanjuan Lv, Chao Gao, Jiayang Qin, Cuiqing Ma*, Zhen Liu, Peihai Liu, Lixiang Li, Ping Xu* (2010) A novel whole-cell biocatalyst with NAD+ regeneration for production of chiral chemicals. PLoS One, 5(1):e8860

  14. Zijun Xiao, Cuiqing Ma, Ping Xu*, Jian R. Lu* (2009) Acetoin catabolism and acetylbutanediol formation by Bacillus pumilus in a chemically defined medium. PLoS One, 4(5):e5627

  15. Zijun Xiao Z, Ping Xu* (2007) Acetoin metabolism in bacteria.Critical Reviews in Microbiology, 33(2):127-140

  16. Z Xiao, PH Liu, JY Qin, P Xu* (2007) Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate.Applied Microbiology and Biotechnology, 74(1):61-68



专利(Patents):
1.
赵静宜, 梁文龙, 肖梓军, 顾如林, 赵玲, 田莉. 生物砂滤池水处理系统. 实用新型专利号ZL 2017207355206, 授权公告日201846

2.肖梓军, 顾如林, 侯孝元, 吕新, 赵静宜. 一株芽孢杆菌及其在高温发酵生产乙偶姻和2,3-丁二醇中的应用. 发明专利号ZL 201610402441.3, 授权公告日2019611

3.赵静宜, 顾如林, 肖梓军, 梁文龙, 赵玲. 一种发酵液中3 羟基丁酮含量的在线检测系统. 实用新型专利号ZL 201720725647X, 授权公告日2018116

4.肖梓军, 侯孝元, 赵静宜, 郗丽君, 李静, 吕建仁. 一种脂肪酶催化合成乙偶姻脂肪酸酯的方法. 发明专利号ZL 201510034086.4,授权公告日20171031

5.肖梓军, 方力, 于洋, 刘海澄, 黄鹤, 相养冬, 朱贤琨, 郗丽君,徐海, 吕建仁. 一株短链脂肪酸降解菌及其应用. 发明专利号ZL 201310608087.6,授权公告日2015318

6.肖梓军, 王相明, 郗丽君, 吕建仁. 一种乙烯废碱液的生化处理工艺方法. 发明专利号ZL 201310303868.4, 授权公告日2015121

7.肖梓军, 黄运玲, 霍方方, 曲剑波, 刘建国, 朱虎. 一株地芽孢杆菌及其在高温发酵生产2,3-丁二醇和乙偶姻中的应用. 发明专利号ZL 201210060900.6,授权公告日20141022

8.肖梓军, 亓瑞倩, 乔树苓, 严勇, 徐海, 吕建仁. 一株高效降解多种酚类化合物的假单胞菌XQ23. 发明专利号ZL 200910019223.1, 授权公告日201266

9.Ping Xu, Zijun Xiao, Zhonghao Wei, Yi Du. Bacillus pumilus strain for high yield of tetramethylpyrazine. 美国专利号US 8,067,212 B2, Nov 29, 2011; 欧洲专利号EP2031052; 中国发明专利号ZL 200610026927.8

10.Ping Xu, Zijun Xiao, Yi Du, Zhonghao Wei. Acetoin high-yield Bacillus pumilus strain. 美国专利号US 8,158,402 B2, Apr 17, 2012; 欧洲专利号EP1820848; 中国发明专利号ZL 200410084381.2

11.Hong Chen, Ping Xu, Zijun Xiao, Yi Du, Yiyong Zeng, Zhaobin Zhang, Qingyi Ma, Zhonghao Wei. A preparation method of butadione by gas-phase oxidating 3-hydroxy-butanone. 欧洲专利号EP1826194, Aug 29, 2007; 中国发明专利号ZL 200410084382.7

12.赵静宜, 马玲燕, 肖梓军, 王霖慧, 董方卉. 5 羟基 4 癸内酯的菌株及应用. 发明专利申请号:201710717804.7

13.肖梓军, 田莉, 赵静宜, 王卓, 董方卉. 一株酵母菌及其在莲子酒发酵中的应用. 发明专利申请号:201910377994.1

14.肖梓军,赵玲,王霖慧,赵静宜. 一株芽孢杆菌及其在制备13C标记的乙偶姻中的应用. 发明专利申请号:201710532433.51.

丰富的活动(Actives



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