蛋白质分子工程实验室

Protein Molecular Engineering Laboratory

Lab website: http://pme.cce.upc.edu.cn/ 

  

Overview

以黄方教授(博导,瑞士巴塞尔大学物理化学专业博士,英国剑桥大学蛋白质工程中心博士后及研究科学家,山东省杰青,教育部新世纪人选,泰山学者海外特聘专家)为学术带头人,目前拥有教授1人,副教授5人,博士后1人,在读博士、硕士研究生20多人。本实验室毕业的本科生、研究生已有多人出国到英国Manchester大学、美国Kansas StateGeorgetown大学、澳大利亚国立大学、德国KarlsruherJacobs大学等国际知名高校攻读硕士或博士学位。

The Protein Molecular Engineering Laboratory, led by Prof. Dr. Fang Huang, has currently 1 professor, 5 associate professors, 1 postdoc and more than20 graduate students. Several students graduated from this lab have entered Manchester University, Kansas State University, Geogetown University, Australian National University, Karlsruher Institute of Technology, and Jacobs University of Bremen, to continue their study.

蛋白质分子工程实验室利用各种现代生物物理技术(特别是单分子荧光技术),对蛋白质折叠机理、与疾病以及能源相关的关键蛋白的功能性质、细胞内吞路径、以及纳米粒子的细胞毒性机理等进行研究,在分子水平上揭示生命的基本过程,并为疾病诊断治疗以及新能源的开发利用提供重要的理论指导。

The Protein Molecular Engineering Laboratory applies various biophysical techniques, particularly single-molecule fluorescence, to investigate the folding mechanism of proteins, the functions and properties of proteins related to diseases and bioenergy, nanomaterials and their applications in imaging and drug delivery. We are trying to understand fundamental life processes at molecular level and to provide theoretical guidance or clues for diseases diagnosis and the discovery of bioenergy.  

Staff

Prof. Dr. Fang HUANG (黄方)

Email:fhuang@upc.edu.cn  Tel.:0532-86981560  

博士生导师

“泰山学者”海外特聘教授

教育部新世纪优秀人才

山东省杰出青年基金获得者

瑞士巴塞尔大学博士,英国剑桥大学研究科学家(Investigator Scientist)

中国化学会生物物理化学专业委员会委员

中国生物物理学会单分子生物学分会理事

Professor and PhD supervisor

PhD from University of Basel, Switzerland (2004)

Postdoc and Investigator Scientist at Cambridge University (2004-2009)

Taishan Scholar

Member of Specialized Committee of Biophysical Chemistry of Chinese Chemical Society

Board member of Single-molecular Biology Division of Chinese Biophysical Society

  

Dr. Daoyong YU (于道永)

       

Email:daoyong@upc.edu.cn   Tel.:0532-86981135

硕士生导师

石油大学(华东)应用化学专业工学博士,2002

美国麻省理工学院生物医学工程中心访问学者,2005-2007

中国化学会、中国化工学会、美国化学会会员

Photosynth. Res.Int. J. Hydrog. Energy应用化学、化工进展等期刊发表论文60余篇

研究方向:能源应用化学

Supervisor for Master Students

PhD from China University of Petroleum (2002)

Visiting ScientistofMassachusettsInstituteofTechnology, USA (2005-2007)

Member of American Chemical Society (2007-), Chinese Chemical Society (2011-) and Chemical Industry and Engineering Society of China(2017-)

Over60 publications on journals including Photosynth. Res., Int. J. Hydrog. Energy,Chinese Journal of Applied Chemistry,andChemical Industry and Engineering Progress

Research direction:Applied Chemistry in Energy


Dr. Xiaojuan WANG (王晓娟)

        

Email:xwang@upc.edu.cn  Tel.:0532-86981560

硕士生导师,应用化学系

瑞士巴塞尔大学博士

剑桥大学博士后

英国高科技上市公司Nanoco研究科学家

青岛市急需拔尖人才引进回国(2011

JACS, ChemComm, AMI等国际期刊发表30多篇SCI论文

研究方向:功能纳米材料,新型生物分析技术

Applied Chemistry Department, Supervisor for Master Students

PhD from University of Basel, Switzerland (2004)

Postdoctoral Researcher of Cambridge University, UK (2005-2009)

Research Scientist of Nanoco Technologies Ltd. Manchester, UK (2009-2011)

Qingdao Urgently Needed Top Talents2011

Over 30 publications on top journals including JACS, ChemComm, and AMI

Research directions:Functional nanomaterials, Novel bioanalysis technologies

  

  

Dr. Baosheng GE (葛保胜)

       

        Email:gebaosheng@upc.edu.cn  Tel.:0532-86981135

硕士生导师

中国科学院海洋研究所海洋生物学专业博士,美国麻省理工学院、俄亥俄州立大学访问学者

主持国家重点计划国际合作重点专项、国家自然科学基金、山东省重点研发计划等项目

发表SCI论文30多篇,申请国家发明专利10项。

研究方向:膜蛋白信号转导分子机制;海洋生物质能源综合利用

Center for Bioengineering and Biotechnology, Supervisor for Master Students

PhD from Institute of Oceanology, CAS (2006)

Visiting Scholars of MIT & The Ohio State University, USA (2007 & 2014)

Undertaking the National key Research and Development Plan of China, National NaturalScience Foundations of China

Over 30 publications and 10 Applied Patents

Research directions:Molecular Mechanisms in Transmembrane Signal Transduction of Membrane Proteins;ComprehensiveUtilization ofMarineBiomassEnergy

  

  

Dr.Hua HE (何化)

Email:huahe@upc.edu.cn  Tel.:0532-86981560  

硕士生导师

上海交通大学应用化学工学博士,美国斯坦福大学访问学者

主持国家自然科学基金、山东省自然科学基金等省部级项目

Angew. Chem. Int. Ed., Anal. Chem.等国际权威SCI刊物发表论文20余篇,论文他引超过600次,单篇最高引用超200

研究方向:生物成像分析;生物分子高灵敏检测与疾病诊断

Supervisor for Master Students

PhD from Shanghai Jiao Tong University, China (2008)

Visiting Scholar from Stanford University, USA (2014-2015)

Undertaking the National Natural Science Foundations of China and Shandong Province

Over 20 publications on top journals includingAngew. Chem. Int. Ed. andAnal. Chem.

Research directions:Biologicalimaging;Biomolecular detection and disease diagnosis

  

Dr. Xiaoqiang WANG (王小强)

        

Email: wangxq001@upc.edu.cnTel.:0532-86981135  

硕士生导师

中国石油大学(华东)博士,美国麻省理工学院联合培养,美国加州大学(戴维斯)访问学者

山东省优秀博士学位论文获得者,

主持国家自然科学基金、省优秀中青年科学家奖励基金等项目

PNAS, AMI等国际权威SCI刊物发表论文20余篇

研究方向:生物能源技术,海洋生物化工

Supervisor for Master students

PhD from China University of Petroleum (East China), jointly trained in Massachusetts Institute of Technology

Visiting scholar of University of California, Davis

Recipient of excellent doctor degree dissertation of Shandong

Funded by National Natural Science Foundation, the Doctoral Foundation of Shandong,et al

Over 20 publications on top journals including PNAS, AMI,et al

Research directions: Bioenergy technology, Marinebiochemical engineering

  

Dr. Xiaoxi YU (于筱溪)

        

Email:yuxiaoxi@upc.edu.cn

化学工程系讲师

天津大学化工学院博士,德国马丁路德大学联合培养博士

主持国家自然科学基金,山东省自然科学基金,中国博士后科学基金等省部级项目

Chem. Eng. Sci.等国际期刊发表论文7

研究方向:生物大分子提纯技术,蛋白质晶体生长

Lecturer fromDepartmentof Chemical Engineering

PhD from Tianjin University,visiting PhD student from Martin Luther University, Halle- Wittenberg, Germany

Conducting several research projects supported by the National Natural Science Foundation of China (NSFC), the Natural Foundation of Shandong and Chinese Postdoctoral Science Foundation

Over7 publications on international journals includingChem. Eng. Sci.

Research directions:novel purification technology of biomarcomolecule,protein crystal growth

  

Research Themes

蛋白质折叠及功能的研究Protein folding and functions

蛋白质的正确折叠是蛋白质实现其功能的必要步骤,折叠机理的问题也是分子生物学中还未完全解答的核心问题之一。另一方面,蛋白质总是通过与其它生物分子的相互作用来实现其功能,而在这些相互作用过程中往往伴随着构象的转变。在这一方向上,我们主要研究蛋白质的折叠机理、蛋白质-蛋白质相互作用、蛋白质-生物膜相互作用、蛋白质实现功能过程中的构象变化等,通过这些研究去阐释蛋白质的构效关系。我们关注的蛋白质主要包括G-蛋白偶联受体、细菌视紫红质、分子伴侣(GroEL)、多态蛋白等。

It is essential to fold properly for proteins to be functional. Protein folding is still one of the standing critical questions in molecular biology. On the other hand, proteins have to interact with other biomolecules to realize their functions, where conformational changes occur. In this direction, we focus on the folding mechanism of proteins, protein-protein interaction, protein-membrane interaction and protein conformational changes, which allows us to reveal the structure-activity relationship of proteins. We are interested in GPCR, bacteriorhodopsin (BR), molecular chaperons (such as GroEL), and metamorphic proteins.

  

  

单分子荧光及超分辨成像技术Single-molecule fluorescence and superresolution imaging

单分子技术能够对单个的蛋白质分子进行分别研究,从而提供系综探测技术所无法获得的信息,在蛋白质折叠及功能研究中具有独特的优势。荧光成像技术可以对活细胞进行实时无损成像,因此在生命科学领域得到广泛应用,但是传统的荧光成像技术却受到光学成像分辨率极限的限制,难以对亚细胞结构进行成像。超分辨荧光成像技术突破光学分辨率极限,可实现10-20纳米的空间分辨率,为生物成像提供了新的手段。本课题组目前正利用单分子荧光技术和超分辨荧光成像技术对蛋白质折叠与功能、蛋白质在细胞膜上的聚集、分布和运动进行研究。

Single-molecule fluorescence can investigate individual single molecules and therefore provide information that is out of reach of ensemble techniques. It has its unique advantages in the study of protein folding and functions. Fluorescence imaging has been broadly applied in life sciences since it can realize non-destructive imaging at real time. However, the resolution of conventional fluorescence microscopy is limited by light diffraction. Superresolution fluorescence imaging can realize a spatial resolution of 10-20 nm and provide a new methodology for bioimaging. We are currently applying single-molecule fluorescence and superresolution imaging to investigate protein folding and functions, protein oligomerization, distribution and moving on cell membrane.

纳米材料的生物应用Biological applications of nanomaterials

纳米材料具有特殊的荧光特性以及穿透细胞膜的能力,因此在细胞染色和药物输送方面得到了广泛的应用。本课题组利用石墨烯量子点、碳点及贵金属纳米簇等新型纳米材料作为特殊荧光探针,用于靶向性的活细胞成像。同时,经过表面修饰这些纳米材料也可作为药物输送载体,实现荧光标记和药物输送的双重功效。

Nanomaterialshave unique fluorescence properties and capability to penetrate cell membrane. They have been widely applied in cell labelling and drug delivery. We are applying graphene quantum dots, carbon dots and noble metal nanoclusters as fluorescence probesfor cell imaging. Upon surface modification, these materials can also be used as drug carrier. We have constructed multi-functional nanomaterials, which can be used for drug delivery and act as fluorescence probes at the same time.

  

微藻生物能源及综合利用Microalgaebioenergy andcomprehensiveutilization

以我国主要经济微藻及其关键蛋白质或酶为研究对象,开展微藻光驱固碳培养、产能代谢调控以及高附加值产品开发等应用基础研究,探索微藻固碳产能的代谢调控机理;并利用基因技术、化学修饰和组装等技术对生物能源直接相关的蛋白和酶(如微藻光合系统中的PSILHC-II、绿藻产氢酶等)进行改造,以调控蛋白在体内和体外的稳定性及活性,从而改善生物能源利用效率、促进生物能源的产业化进程;此外,还开发藻蓝蛋白、小分子活性寡肽等多种微藻生物活性产品,从而为我国海洋微藻的产业化推进奠定重要的理论和技术基础。

Taking China's main economic microalgae and its key proteins or enzymes as research objects, a series of basic research, such as light-driving carbon fixation by microalgae, metabolic regulation to improveenergyproduction, and high-value-added product development, are conducted to explore the metabolic regulation mechanism of microalgal carbon fixation and biomass production. In addition, technologies such as genetics, chemical modification, and assembly are used to modify bioenergy-related proteins and enzymes (such as microalgae photosynthetic complexes of PSI and LHC-II, and hydrogenase from green algae, etc.), to enhance their stability and activityin vivo andin vitro, thereby improving the efficiency of bioenergy utilization, and promoting the industrialization of bioenergy. Further, we develop a variety of microalgal products with bioactivity (such as phycocyanin, small molecule peptides, etc.), and lay an important theoretical and technological foundation for the industrialization of marine microalgae in China.

  

Selected Publications

  

Wang, X. et al. Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells.ACS Appl. Mater. Interfaces9, 17799-17806 (2017).

Wang, X. et al. Chaperonin-Nanocaged Hemin as an Artificial Metalloenzyme for Oxidation Catalysis.ACS Appl. Mater. Interfaces9, 25387-25396 (2017).

3  Lao, J. et al. Single-Molecule Imaging Demonstrates Ligand Regulation of the Oligomeric Status of CXCR4 in Living Cells.J. Phys. Chem. B121, 1466-1474 (2017).

He, H. et al. High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots.Anal. Chem.89, 11831-11838 (2017).

Ge, B. et al. Single-molecule imaging reveals dimerization/oligomerization of CXCR4 on plasma membrane closely related to its function.Sci. Rep.7, 16873 (2017).

Zhao, Y. et al. Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state.Protein Eng. Des. Sel.29, 23-29 (2016).

Wang, X. et al. Active tumor-targeting luminescent gold clusters with efficient urinary excretion.Chem. Commun.52, 9232-9235 (2016).

He, H. et al. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4.Chem.--Eur. J.22, 5863-5867 (2016).

Chi, H., Wang, X., Li, J., Ren, H. & Huang, F. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES.Sci. Rep.5, 17037 (2015).

10 Yu, D. et al. Effect of surfactants on apparent oxygen consumption of photosystem I isolated fromArthrospira platensis.Photosynth Res.122, 203-213 (2014).